Department of Microbiology

Notice

News

Event

Details of Dr. Md. Mizanur Rahaman


 

BIOGRAPHICAL SKETCH

CURRENT POSITION

Lecturer

Department of Microbiology

University of Dhaka

Dhaka, Bangladesh

Phone: +8801796585290(cell), Email: razu002@yahoo.com

EDUCATION/TRAINING

INSTITUTION AND LOCATION

DEGREE

MM/YY

FIELD OF STUDY

University of Dhaka, Bangladesh

B.Sc.

2004 (p 2006)

Microbiology

University of Dhaka, Bangladesh

M.S.

2005 (p 2008)

Microbiology

Kumamoto University, Japan

PhD

2013

Microbiology and Medical Science

University of Pittsburgh, PA, USA

Postdoctoral

2013-2015

Molecular Signaling and Cardiovascular Medicine

Tohoku University, Japan

 

University of Dhaka

Assistant Professor

Lecturer

2015

 

2015

Department of Environmental Health Sciences and Molecular Toxicology

Microbiology

 

A. Career Goal

To carry out top quality research as an independent researcher and become a successful academician, that provides opportunity to use my scientific and academic knowledge with expertize and contribute in Science and for the Country.

B. Position and Honors

Positions and Employment

2006-2008

M.S. Research Student, Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.

2006-2008

Research Student, International Center for Diarrheal Disease Research, ICDDR,B , Enteric & Food Microbiology laboratory, Dhaka, Bangladesh

2008-2009

Senior Research Assistant, International Center for Diarrheal Disease Research, ICDDR,B , Enteric & Food Microbiology laboratory, Dhaka, Bangladesh

2009

Research Officer, International Center for Diarrheal Disease Research, ICDDR,B , Enteric & Food Microbiology laboratory, Dhaka, Bangladesh

2009-2013

Research Assistant, Kumamoto University Medical Sciences, Department of Microbiology Kumamoto, Japan

2009-2013

Teacher Assistant, Kumamoto University Medical Sciences, Department of Microbiology Kumamoto, Japan

2013-2015

2015

 

2015

Postdoctoral Researcher, Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Assistant Professor, Department of Environmental Health Sciences and Molecular Toxicology, Tohoku University, Japan

Lecturer, Department of Microbiology, University of Dhaka.

 

Honors

 

2009

Japanese Government Scholarship (Monbukagakusho) for PhD program, Kumamoto University Graduate School of Medical Sciences, Japan.

2012

Koichi Suzuki Memorial Award for best poster (85th Annual Meeting of the Japanese Biochemical Society, Fukuoka, Japan)

2012

2014

Young investigator award for 23rd Life Style and stress signaling conference, Kumamoto, Japan

American Heart Association Fellowship grant for postdoctoral research

C. Professional Societies Membership

2011-2013

Member, Japanese biochemical Society.

2008-2009

Member, Bangladesh Society of Microbiologists (BSM), Bangladesh.

2009-Pres

2013-Pres

2013-Pres

2013-Pres

Life member, Graduate Microbiologist Association (GMA), Bangladesh.

Member of Nitric Oxide Society

Member of SFRBM

Member of American Heart Association

D. Academic Teaching:

During my Postdoctoral I used to teach molecular signal transduction and immunology courses at University of Pittsburgh.

During my stay at Tohoku University, Japan as an Assistant Professor I used to teach Molecular toxicology, Metal and Environmental Pollution and Innate immunity courses to undergraduate students.

E. Selected Peer-Reviewed Publications

  1. Akashi S, Ahmed KA, Sawa T, Ono K, Tsutsuki H, Burgoyne JR, Ida T, Horio E, Prysyazhna O, Oike Y, Rahaman MM, Eaton P, Fujii S, Akaike T. Persistent Activation of cGMP-Dependent Protein Kinase by a Nitrated Cyclic Nucleotide via Site Specific Protein S-Guanylation. Biochemistry, 2016 Feb 9;55(5):751-61. doi:10.1021/acs.biochem.5b00774. (Imfact Factor: 3.015)

2.     Rahaman MM, Reinders FG, Koes D,  Nguyen AT, Mutchler SM, Watkins CS, Alvarez RA, Miller MP, Cheng D, Chen BB, Jackson EK, Camacho CJ and Straub AC. Structure guided chemical    modifications of propylthiouracil reveal small molecule inhibitors of cytochrome b5 reductase 3 that increase nitric oxide bioavailability.(Journal of Biological Chemistry 2015 May 22. pii: jbc.M114.629964. (Impact Factor: 4.6)

3.     Rahaman MM, Straub AC. The emerging roles of somatic globins in cardiovascular redox biology and beyond. Redox Biol. 2013; 1(1): 405-410. Review. (Impact Factor: 3.6)

4.     Rahaman MM, Sawa T, Ahmed KA, Khan S, Inoue H, Irie A, Fujii S, and Akaike T. S- Guanylation Proteomics for Redox-based Mitochondrial Signaling. Antioxidants and Redox Signaling, 2012 Sep 16 (doi: 10.1089/ars.2012.4606) (Impact Factor: 8.3)

5.     Khan S, Rahman HN, Okamoto T, Matsunaga T, Fujiwara Y, Sawa T, Yoshitake J, Ono K, Ahmed KA, Rahaman MM, Oyama K, Takeya M, Ida T, Kawamura Y, Fujii S, and Akaike T. Promotion of atherosclerosis by Helicobacter cinaedi infection that involves macrophage-driven proinflammatory responses. Scientific Reports, 15(4): 4680, (doi: 10.1038/srep04680.) April 2014 (Impact Factor: 5.1)

6.     Talukder PK, Rahaman MM, Rahman M, Nabi A, Islam Z, Hoque MM, Endtz        HP, Islam MA. Antimicrobial resistance, virulence factors and genetic diversity of Escherichia coli isolates from household water supply in Dhaka, Bangladesh. PLoS One. 2013; 8(4): e61090. (Impact Factor: 3.9)

7.     Ahmed KA, Sawa T, Ihara H, Kasamatsu S, Yoshitake J, Rahaman MM, Okamoto T, Fujii S, Akaike T. Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP: potential implications for ROS signalling. Biochem J. 2012; 441(2): 719-30. (Impact Factor: 4.8)

8.   Rahaman MM, Rahman ZM, Begum A, Akhter H, Khan SI. Occurrence and characterization of Vibrio spp in raw and frozen shrimps. Bangladesh J. Zool. 2009; 37(2): 247-255.

Manuscripts under revision

1.     Rahaman MM, Sawa T, Fujii S, Ono K, and Akaike T. Electrophilic activites of guanosine and aromatic amino acid derivatives formed under oxidative stress conditions. (Research aricle)

2.   Rahaman MM, Sawa T, Ono K, Ida T,  Fujii S, Ihara H, and Akaike T. Substrate efficiency of guanylated cyclase towards GTP and modified GTP analogs under oxidative stress conditions. (Research aricle)

Abstracts and Presentations

1.   Rahaman MM, Reinders F, Watkins CE, Miller MP, Gladwin MT, Koes D, Camacho CJ and Straub AC.  Structure guided chemical modifications of propylthiouracil reveal novel small molecule inhibitors of cytochrome b5 reductase 3  (8th International Conference on the biology, chemistry and therapeutic application of nitric Oxide, Cleveland USA, June 16-20, 2014)

2.   Rahaman MM, Sawa T, Ahmed KA, Khan S, Inoue H, Irie A, Fujii S, and Akaike T. S-Guanylation proteomics for redox-based mitochondrial signaling. (The 85th Annual Meeting of the Japanese Biochemical Society, December 14-16, Fukuoka, 2012)

3.   Rahaman MM, Sawa T, Ahmed KA, Khan S, Inoue H, Irie A, Fujii S, and Akaike T. S-Guanylation proteomics for redox-based mitochondrial signaling. (The 12th Annual Scientific Meeting of the Nitric oxide Society of Japan, June 29-30, 2012, Kobe, Japan)

4.   Rahaman MM, Sawa T, Okamoto T, Fujii S, Ono K and Akaike T. Nitrated nucleotides reaction with biological low molecular weight sulfhydryls: kinetic analysis. (11th Annual Scientific Meeting of the NO-society of Japan, 2011,  Machida, Tokyo, Japan)

5.   Rahaman MM, Sawa T, Okamoto T, Fujii S, Ono K and Akaike T. Structure-activity analysis of nitrated nucleotides as electrophiles for reaction with sulfhydryls. (The 84th Annual Meeting of the Japanese Biochemical Society, September 21-24, 2011, Kyoto, Japan)

6.   Rahaman MM, Sawa T, Okamoto T, Fujii S, Ono K and Akaike T. Proteomic identification for sulfhydryl modifications of mitochondrial proteins by 8-nitroguanosine 3',5'-cyclic monophosphate. (The 6th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric oxide, June14-18, 2010, Kyoto, Japan) 

F. Present Research

I am investigating the inflammasome activation and inflammatory diseases especially inflammatory bowel diseases.

G. Previous Research

I used to investigate the redox-controlled cell signaling mechanisms that regulate endothelial and smooth muscle biology of the microcirculation in the setting of both physiology and cardiovascular disease. I also worked to identify the molecular mechanisms involved in redox signaling in microcirculation. Very recently, we have identified a specific reductase protein is critically involved in redox regulation of nitric oxide signaling. We have used retro-viral shRNA to knockdown the specific protein in different cell types and found significant effect of the protein. We also have developed mutant proteins by genetic manipulation and found significant impact of the mutation.

In one of my recent work I have developed small molecule inhibitors against a very important reductase enzyme using computational chemistry with a very effective IC50 value. This is the first step towards developing small molecule inhibitors and ultimately potential drug candidates to treat hypertension. Then I was working on to identify unique roles of Cytochrome B5 reductase 3 in the vasculature to understand more details of its regulatory effects and finally to control/treat hypertension and blood pressure.  

H. Grant and manuscript writing skills

I had successfully submitted some grants provided by Dhaka University and different minstries in Bangladesh. 

I had successfully submitted AHA fellowship Grant (University of Pittsburgh, PA, USA, 2014) which is a highly competitive prestigious award all over America. I had also helped my previous supervisor to write 2 RO1 Grant for NIH. So far I have published 8 research and review articles and 4 more articles are in pipeline for publications. I had a very good training for peer reviewed scientific paper writing as well as grants writing.

I. Scientific reviewing skills

I have veenworking as a scientific reviewer for scientific articles for 2 years for the journal of ‘Nitric Oxide’ and journal of “PlosOne’

J. Discovery Patents

I had applied for the patent of novel small molecule inhibitors of Cytochrome B5 reductase 3 from the Innovation center of University of Pittsburgh.

K. PhD Research

In my graduate study at the Department of Microbiology, Graduate School of Medical Sciences, Kumamoto University, Japan, I had studied the roles of reactive oxygen species (ROS) in the formation of 8-nitroguanosine 3', 5'-cyclic monophosphate (8-nitro-cGMP) and endogenous mitochondrial protein S-guanylation in the cellular milieu under the oxidative stress condition.

1. 8-Nitro-cGMP is a nitrated derivative of cGMP that is formed via cross-talk of reactive oxygen species formed by NADPH oxidase 2 and mitochondria. This nitrated nucleotide can function as a unique electrophilic second messenger in regulation of redox signaling by inducing a post-translational modification of protein thiols via cGMP adduction (protein S-guanylation). With S-guanylation proteomics, I investigated endogenous mitochondrial protein S-guanylation. I had developed a new mass spectrometry (MS)-based proteomic method—S-guanylation proteomics—which comprised two approaches: (i) direct protein digestion followed by immunoaffinity capture of S-guanylated peptides that were subjected to liquid chromatography-tandem MS (LC-MS/MS), and (ii) 2D-gel electrophoretic separation of S-guanylated proteins that were subjected to in-gel digestion, followed by LC-MS/MS. We thereby identified certain mitochondrial proteins that are S-guanylated endogenously during immunological stimulation, including mortalin and 60-kDa heat shock protein (HSP60). Mortalin and HSP60 were recently reported to regulate mitochondrial permeability transition pore (mPTP) opening, at least partly, by interacting with cyclophilin D, an mPTP component. Our data revealed that immunological stimulation and 8-nitro-cGMP treatment induced mPTP opening in a cyclophilin D-dependent manner. Our S-guanylation proteomic method determined that mitochondrial heat shock proteins may be novel targets for redox modification via protein S-guanylation that participates in mPTP regulation and mitochondrial redox signaling.

This particular work has been published in Antioxidants and Redox Signaling, 2012 Sep 16 (doi: 10.1089/ars.2012.4606)

2. I have also worked on the electrophilicity of 8-nitro-cGMP and related derivatives with biological low molecular weight sulfhydryls (LMW-SHs) including cysteine, homocysteine and glutathione (GSH) and selenocysteine.  Later I had worked on to determine the effect of these compounds in the inflammation. In brief macrophage derived from php1 monocytes were treated with these compounds followed by stimulated with LPS and IFN gamma to measure the level of different cytokines by real time PCR and also ELISA. This work has been completed and submitted to Journal of Biological Chemistry for publication.

3. One of my major work was to determine enzymatic kinetics of guanylated cyclase.  To determine the kinetic parameters (Km, Vmax and Kcat)  of guanylate cyclase with GTP and modified GTPs for example 8-nitro-GTP, 8-oxo-GTP, 8-Br-GTP I had used 3 different kind of guanylated cyclase including recombinant Chlamydomonas guanylate cyclase, soluble guanylated cyclase from bovine and recombinant particulate guanylated cyclase. I have already finished the study and submitted to Journal of Biological Chemistry for publication.

L.Technical skills in different field

Laser Microscope imaging

Confocal laser microscope handling with Nikon EZ-C1

Animal handling

       Mice handling and small animal surgeries for implantation of osmotic pump, organ isolation, Aorta isolation for myography etc.

Blood Pressure study

  • Pressure myography
  • Animal blood pressure measurement by telemetric assay.         

Immunology

  •  FACS analysis
  •   Western blot analysis
  •   ELISA
  •   Immunoaffinity purifications of proteins and nanomolecules
  •   Immunocytochemistry
  •    Immunohistochemistry 


Cell biology

  • Handling and maintenance of different mammalian cells including, Rat C6 glial cells, Rat smooth   muscle cells, Human smooth muscle cells, Endothelial Cells, Mouse Macrophage cells, RAW cells, Hella cells
  •  Confocal laser microscope handling (Nikon EZ-C1)
  • Fluorescent microscope handling 

Molecular biology

  • DNA and RNA various preparation methods,
  • PCR, RT-PCR, real time PCR, 

  • Gene silencing using si-RNA, sh-RNA
  • Mammalian cell transfection (lipofectamine based methods)
  • Retroviral gene transfection

  • Apoptosis detecting analyses

Protein-protein interaction

  • Immuno pull down or co-precipitation of proteins.
  • Proximity Ligation Assay (PLA)

Proteomics

LC-MS/MS detection of proteins by MASCOT search
- 2D gel electrophoresis
- Silver staining
- Protein isolation from 2D gel and purification for proteomics analysis

Quantitative analysis

  • HPLC- PDA detection and purification of nano molecules
  • HPLC-ECD detection of nanomolecules in subnanomolar level
  • Mass spectrometry for the detection of nanomolecules (Agilent Technologies 6430 Triple 
Quad
  •  LC/MS)
  • Proteomics analysis by Agilent technology Q-TOF LC/MS
  • Stable isotope quantification of biological materials by LC-MS/MS analysis.

Free radical biology and in vitro imaging

  • Reactive oxygen species detection in cells by fluorescent staining, and FACS analysis
  • Mitochondrial superoxide detection by fluorescent imaging
  • Cellular hydrogen peroxide detection by fluorescent method
  • Pegylation of Enzymes, Superoxide dismutase, Catalase
  • Total cell and Mitochondrial SOD activity assay
  • Nitric oxide detection by Griess method 


M.  Language Skills

  • Bengali: Mother tongue
  • English (Reading/Writing/Speaking/Understanding) fluent
  • Japanese (Speaking/Understanding) Fluent

N. Extra curricular activities

  • Key role in organizing the 6th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric oxide, June14-18, 2010, Kyoto, Japan.
  • Soccer player of Vascular Medicine Institute, University of Pittsburgh, USA and previously of the Department of Microbiology, University of Dhaka, Bangladesh.
  • Social charity activities during natural disaster situation, i.e., Oral saline project, during flood time in Bangladesh, 1998; During Tsunami in Japan, 2013.
  • Member of nature Study club, Notre-dame College, Dhaka, Bangladesh

O. Hobbies

  • Photography, Traveling, Playing Cricket, Reading Newspaper, Listening Music, Fishing & Boating.