Profile of Dr. Md. Mizanur Rahaman

... Name Dr. Md. Mizanur Rahaman
Designation Assistant Professor
Last Degree PhD in Medical Science (Japan), MS in Microbiology (DU), BSc in Microbiology (DU)
   

INSTITUTION AND LOCATION

DEGREE

MM/YY

FIELD OF STUDY

University of Dhaka, Bangladesh

B.Sc.

2004 (p 2006)

Microbiology

University of Dhaka, Bangladesh

MS

2005 (p 2008)

Microbiology

Kumamoto University, Japan

PhD

2013

Microbiology and Medical Science

University of Pittsburgh, Pennsylvenia, USA

Postdoctoral

2013-2015

Molecular Signaling and Cardiovascular Medicine

 

2006-2008

M.S. Research Student, Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.

2006-2008

Research Student, International Center for Diarrheal Disease Research, ICDDR,B , Enteric & Food Microbiology laboratory, Dhaka, Bangladesh

2008-2009

Research Officer, International Center for Diarrheal Disease Research, ICDDR,B , Enteric & Food Microbiology laboratory, Dhaka, Bangladesh

2009-2013

Research Assistant, Kumamoto University Medical Sciences, Department of Microbiology Kumamoto, Japan

2009-2013

Teacher Assistant, Kumamoto University Medical Sciences, Department of Microbiology Kumamoto, Japan

2013-2015

 

Postdoctoral Researcher, Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

2015

 

Assistant Professor, Department of Environmental Health Sciences and Molecular Toxicology

Tohoku University, Japan

2015 - Present

Faculty member, Department of Microbiology, University of Dhaka.

A. Selected Peer-Reviewed Publications

 

  1. Rahaman MM, Nguyen AT, Miller MP, Hahn SA, Sparacino-Watkins C, Jobbagy S, Carew NT, Cantu-Medellin N, Wood KC, Baty C, Schopfer FJ, Kelley EE, Gladwin MT, Martin E and Straub AC. Cytochrome b5 Reductase 3 Modulates Soluble Guanylate Cyclase Redox State and cGMP Signaling. Circ Res. 2017 Jun 5. pii: CIRCRESAHA.117.310705. doi: 10.1161/CIRCRESAHA.117.310705.

 

  1. Hossain MB, Mahbub NDB, Chowdhury MMK and Rahaman MM.  Molecular characterization of Enterobacter and Escherichia coli pathotypes prevalent in the popular street foods of Dhaka city and their multidrug resistance. Bangladesh J Microbiol, Volume 35, Number 2, December 2017, pp 11-15

 

  1. Hassan MM, Chakrabarty RP, Siddique MA and Rahaman MM. Prevalence of antibiotic resistant enteric bacteria in the hands of street food vendors in Dhaka city. Bangladesh J Microbiol. Volume 34, Number 1, June 2017, pp 33-38

 

  1. Mukti RF, Chowdhury MMK, Nahid MA, Rahaman MM and Uddin MA. Antibacterial Activity and Physicochemical Properties of Florally Diversified Bangladeshi Honeys. Bangladesh J Microbiol, Volume 33, Number 1&2, June-December 2016, pp 05-09

 

  1. Akashi S, Ahmed KA, Sawa T, Ono K, Tsutsuki H, Burgoyne JR, Ida T, Horio E, Prysyazhna O, Oike Y, Rahaman MM, Eaton P, Fujii S and Akaike T. Persistent Activation of cGMP-Dependent Protein Kinase by a Nitrated Cyclic Nucleotide via Site Specific Protein S-Guanylation. Biochemistry. 2016 Feb 9;55(5):751-61.

 

  1. Rahaman MM, Reinders FG, Koes D,  Nguyen AT, Mutchler SM, Watkins CS, Alvarez RA, Miller MP, Cheng D, Chen BB, Jackson EK, Camacho CJ and Straub AC. Structure guided chemical    modifications of propylthiouracil reveal small molecule inhibitors of cytochrome b5 reductase 3 that increase nitric oxide bioavailability.(Journal of Biological Chemistry 2015 May 22. pii: jbc.M114.629964.

 

  1. Rahaman MM, Straub AC. The emerging roles of somatic globins in cardiovascular redox biology and beyond. Redox Biol. 2013; 1(1): 405-410. Review.

 

  1. Rahaman MM, Sawa T, Ahmed KA, Khan S, Inoue H, Irie A, Fujii S, and Akaike T. S- Guanylation Proteomics for Redox-based Mitochondrial Signaling. Antioxidants and Redox Signaling, 2012 Sep 16 (doi: 10.1089/ars.2012.4606)

 

  1. Khan S, Rahman HN, Okamoto T, Matsunaga T, Fujiwara Y, Sawa T, Yoshitake J, Ono K, Ahmed KA, Rahaman MM, Oyama K, Takeya M, Ida T, Kawamura Y, Fujii S, and Akaike T. Promotion of atherosclerosis by Helicobacter cinaedi infection that involves macrophage-driven proinflammatory responses. Scientific Reports, 2014 15(4): 4680.

 

  1. Talukder PK, Rahaman MM, Rahman M, Nabi A, Islam Z, Hoque MM, Endtz             HP, Islam MA. Antimicrobial resistance, virulence factors and genetic diversity of Escherichia coli isolates from household water supply in Dhaka, Bangladesh. PLoS One. 2013; 8(4): e61090.

 

  1. Ahmed KA, Sawa T, Ihara H, Kasamatsu S, Yoshitake J, Rahaman MM, Okamoto T, Fujii S, Akaike T. Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP: potential implications for ROS signalling. Biochem J. 2012; 441(2): 719-30.

 

  1. Rahaman MM, Rahman ZM, Begum A, Akhter H, Khan SI. Occurrence and characterization of Vibrio spp in raw and frozen shrimps. Bangladesh J. Zool. 2009; 37(2): 247-255.    

 

B. Manuscripts under revision

 

  1. Rahaman MM, Sawa T, Fujii S, Ono K, and Akaike T. Electrophilic activites of guanosine and aromatic amino acid derivatives formed under oxidative stress conditions. (Research aricle)
  2. Rahaman MM, Sawa T, Ono K, Ida T,  Fujii S, Ihara H, and Akaike T. Substrate efficiency of guanylated cyclase towards GTP and modified GTP analogs under oxidative stress conditions.

 

C. Abstracts and Presentations

 

1.   Rahaman MM, Reinders F, Watkins CE, Miller MP, Gladwin MT, Koes D, Camacho CJ and Straub AC.  Structure guided chemical modifications of propylthiouracil reveal novel small molecule inhibitors of cytochrome b5 reductase 3  (8th International Conference on the biology, chemistry and therapeutic application of nitric Oxide, Cleveland USA, June 16-20, 2014)

2.   Rahaman MM, Sawa T, Ahmed KA, Khan S, Inoue H, Irie A, Fujii S, and Akaike T. S-Guanylation proteomics for redox-based mitochondrial signaling. (The 85th Annual Meeting of the Japanese Biochemical Society, December 14-16, Fukuoka, 2012)

3.   Rahaman MM, Sawa T, Ahmed KA, Khan S, Inoue H, Irie A, Fujii S, and Akaike T. S-Guanylation proteomics for redox-based mitochondrial signaling. (The 12th Annual Scientific Meeting of the Nitric oxide Society of Japan, June 29-30, 2012, Kobe, Japan)

4.   Rahaman MM, Sawa T, Okamoto T, Fujii S, Ono K and Akaike T. Nitrated nucleotides reaction with biological low molecular weight sulfhydryls: kinetic analysis. (11th Annual Scientific Meeting of the NO-society of Japan, 2011,  Machida, Tokyo, Japan)

5.   Rahaman MM, Sawa T, Okamoto T, Fujii S, Ono K and Akaike T. Structure-activity analysis of nitrated nucleotides as electrophiles for reaction with sulfhydryls. (The 84th Annual Meeting of the Japanese Biochemical Society, September 21-24, 2011, Kyoto, Japan)

6.   Rahaman MM, Sawa T, Okamoto T, Fujii S, Ono K and Akaike T. Proteomic identification for sulfhydryl modifications of mitochondrial proteins by 8-nitroguanosine 3',5'-cyclic monophosphate. (The 6th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric oxide, June14-18, 2010, Kyoto, Japan)

Currently I am teaching different undergraduate and MS courses including General Microbiology, Cell Biology, Biochemistry, Human Physiology, Fermentation technology, Enzyme and Protein Technology at the Department of Microbiology, University of Dhaka.

2009

Japanese Government Scholarship (Monbukagakusho) for PhD program, Kumamoto University Graduate School of Medical Sciences, Japan.

2012

Koichi Suzuki Memorial Award for best poster (85th Annual Meeting of the Japanese Biochemical Society, Fukuoka, Japan)

2012

Young investigator award for 23rd Life Style and stress signaling conference, Kumamoto, Japan

2014

American Heart Association Fellowship grant for postdoctoral research

Currently I am working on different research projects including understanding of different antibiotic resistance mechanisms, novel antibiotics from different natural and innovative sources, Host-pathogen interaction analysis especially activation of inflammasome by bacteria. I am also working on research projects for prevention of coronary artery blockages. 

Currently I am teaching different undergraduate and MS courses including General Microbiology, Cell Biology, Biochemistry, Human Physiology, Fermentation technology, Enzyme and Protein Technology at the Department of Microbiology, University of Dhaka.

Biographical Sketch of

MD. MIZANUR RAHAMAN, PhD

 

CURRENT POSITION

Assistant Professor

Department of Microbiology

University of Dhaka

Dhaka, Bangladesh

Phone: +8801796585290(cell), Email: razu002@du.ac.bd

EDUCATION

INSTITUTION AND LOCATION

DEGREE

MM/YY

FIELD OF STUDY

University of Dhaka, Bangladesh

B.Sc.

2004 (p 2006)

Microbiology

University of Dhaka, Bangladesh

M.S.

2005 (p 2008)

Microbiology

Kumamoto University, Japan

PhD

2013

Microbiology and Medical Science

University of Pittsburgh, PA, USA

Postdoctoral

2013-2015

Molecular Signaling and Cardiovascular Medicine

 

 

 

 

 

 

 

  1. Position and Honors

 

Positions and Employment

 

2006-2008

M.S. Research Student, Department of Microbiology, University of Dhaka, Dhaka, Bangladesh.

2006-2008

Research Student, International Center for Diarrheal Disease Research, ICDDR,B , Enteric & Food Microbiology laboratory, Dhaka, Bangladesh

2008-2009

Research Officer, International Center for Diarrheal Disease Research, ICDDR,B , Enteric & Food Microbiology laboratory, Dhaka, Bangladesh

2009-2013

Research Assistant, Kumamoto University Medical Sciences, Department of Microbiology Kumamoto, Japan

2009-2013

Teacher Assistant, Kumamoto University Medical Sciences, Department of Microbiology Kumamoto, Japan

2013-2015

 

Postdoctoral Researcher, Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

2015

 

Assistant Professor, Department of Environmental Health Sciences and Molecular Toxicology

Tohoku University, Japan

2015 - Present

Faculty member, Department of Microbiology, University of Dhaka.

 

Honors

 

2009

Japanese Government Scholarship (Monbukagakusho) for PhD program, Kumamoto University Graduate School of Medical Sciences, Japan.

2012

Koichi Suzuki Memorial Award for best poster (85th Annual Meeting of the Japanese Biochemical Society, Fukuoka, Japan)

2012

Young investigator award for 23rd Life Style and stress signaling conference, Kumamoto, Japan

2014

American Heart Association Fellowship grant for postdoctoral research

 

  1. Professional Societies Membership

 

 

2011-2013

Member, Japanese Biochemical Society.

2008-2009

Member, Bangladesh Society of Microbiologists (BSM), Bangladesh.

2009-Pres

Life member, Graduate Microbiologist Association (GMA), Bangladesh.

2013-Pres

Member of Nitric Oxide Society

2013-Pres

Member of SFRBM

2013-Pres

Member of American Heart Association

2016-Pres

Member, American Society of Microbiologists (ASM)

 

  1. Academic Teaching:
  • Currently I am teaching different undergraduate and MS courses including Cell Biology, Biochemistry, Human Physiology, Fermentation technology, Enzyme and Protein Technology at the Department of Microbiology, University of Dhaka.
  • During my Postdoctoral I used to teach molecular signal transduction and immunology courses at University of Pittsburgh.
  • During my stay at Tohoku University, Japan as an Assistant Professor I used to teach Molecular toxicology, Metal and Environmental Pollution and Innate immunity courses to undergraduate students.

 

  1. Selected Publications

a. Peer-Reviewed Journal Articles

  1. Rahaman MM, Nguyen AT, Miller MP, Hahn SA, Sparacino-Watkins C, Jobbagy S, Carew NT, Cantu-Medellin N, Wood KC, Baty C, Schopfer FJ, Kelley EE, Gladwin MT, Martin E and Straub AC. Cytochrome b5 Reductase 3 Modulates Soluble Guanylate Cyclase Redox State and cGMP Signaling. Circ Res. 2017 Jun 5. pii: CIRCRESAHA.117.310705. doi: 10.1161/CIRCRESAHA.117.310705. 
  1. Hossain MB, Mahbub NDB, Chowdhury MMK and Rahaman MM.  Molecular characterization of Enterobacter and Escherichia coli pathotypes prevalent in the popular street foods of Dhaka city and their multidrug resistanceBangladesh J Microbiol, Volume 35, Number 2, December 2017, pp 11-15 
  1. Hassan MM, Chakrabarty RP, Siddique MA and Rahaman MM. Prevalence of antibiotic resistant enteric bacteria in the hands of street food vendors in Dhaka city. Bangladesh J MicrobiolVolume 34, Number 1, June 2017, pp 33-38 
  1. Mukti RF, Chowdhury MMK, Nahid MA, Rahaman MM and Uddin MA. Antibacterial Activity and Physicochemical Properties of Florally Diversified Bangladeshi Honeys. Bangladesh J Microbiol, Volume 33, Number 1&2, June-December 2016, pp 05-09 
  1. Akashi S, Ahmed KA, Sawa T, Ono K, Tsutsuki H, Burgoyne JR, Ida T, Horio E, Prysyazhna O, Oike Y, Rahaman MM, Eaton P, Fujii S and Akaike T. Persistent Activation of cGMP-Dependent Protein Kinase by a Nitrated Cyclic Nucleotide via Site Specific Protein S-Guanylation. Biochemistry. 2016 Feb 9;55(5):751-61. 
  1. Rahaman MM, Reinders FG, Koes D,  Nguyen AT, Mutchler SM, Watkins CS, Alvarez RA, Miller MP, Cheng D, Chen BB, Jackson EK, Camacho CJ and Straub AC. Structure guided chemical    modifications of propylthiouracil reveal small molecule inhibitors of cytochrome b5 reductase 3 that increase nitric oxide bioavailability.(Journal of Biological Chemistry 2015 May 22. pii: jbc.M114.629964. 
  1. Rahaman MM, Straub AC. The emerging roles of somatic globins in cardiovascular redox biology and beyond. Redox Biol.2013; 1(1): 405-410. Review. 
  1. Rahaman MM, Sawa T, Ahmed KA, Khan S, Inoue H, Irie A, Fujii S, and Akaike T. S- Guanylation Proteomics for Redox-based Mitochondrial Signaling. Antioxidants and Redox Signaling2012 Sep 16 (doi: 10.1089/ars.2012.4606) 
  1. Khan S, Rahman HN, Okamoto T, Matsunaga T, Fujiwara Y, Sawa T, Yoshitake J, Ono K, Ahmed KA, Rahaman MM, Oyama K, Takeya M, Ida T, Kawamura Y, Fujii S, and Akaike T. Promotion of atherosclerosis by Helicobacter cinaedi infection that involves macrophage-driven proinflammatory responses. Scientific Reports, 2014 15(4): 4680. 
  1. Talukder PK, Rahaman MM, Rahman M, Nabi A, Islam Z, Hoque MM, Endtz             HP, Islam MA. Antimicrobial resistance, virulence factors and genetic diversity of Escherichia coli isolates from household water supply in Dhaka, Bangladesh. PLoS One. 2013; 8(4): e61090. 
  1. Ahmed KA, Sawa T, Ihara H, Kasamatsu S, Yoshitake J, Rahaman MM, Okamoto T, Fujii S, Akaike T. Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP: potential implications for ROS signalling. Biochem J. 2012; 441(2): 719-30. 
  1. Rahaman MM, Rahman ZM, Begum A, Akhter H, Khan SI. Occurrence and characterization of Vibrio spp in raw and frozen shrimps. Bangladesh J. Zool. 2009; 37(2): 247-255. 

 

b. Manuscripts under revision

  1. Rahaman MM, Sawa T, Fujii S, Ono K, and Akaike T. Electrophilic activites of guanosine and aromatic amino acid derivatives formed under oxidative stress conditions. (Research aricle)
  2. Rahaman MM, Sawa T, Ono K, Ida T,  Fujii S, Ihara H, and Akaike T. Substrate efficiency of guanylated cyclase towards GTP and modified GTP analogs under oxidative stress conditions.

 

c. Abstracts and Presentations

1.   Rahaman MM, Reinders F, Watkins CE, Miller MP, Gladwin MT, Koes D, Camacho CJ and Straub AC.  Structure guided chemical modifications of propylthiouracil reveal novel small molecule inhibitors of cytochrome b5 reductase 3  (8th International Conference on the biology, chemistry and therapeutic application of nitric Oxide, Cleveland USA, June 16-20, 2014)

2.   Rahaman MM, Sawa T, Ahmed KA, Khan S, Inoue H, Irie A, Fujii S, and Akaike T. S-Guanylation proteomics for redox-based mitochondrial signaling. (The 85th Annual Meeting of the Japanese Biochemical Society, December 14-16, Fukuoka, 2012)

3.   Rahaman MM, Sawa T, Ahmed KA, Khan S, Inoue H, Irie A, Fujii S, and Akaike T. S-Guanylation proteomics for redox-based mitochondrial signaling. (The 12th Annual Scientific Meeting of the Nitric oxide Society of Japan, June 29-30, 2012, Kobe, Japan)

4.   Rahaman MM, Sawa T, Okamoto T, Fujii S, Ono K and Akaike T. Nitrated nucleotides reaction with biological low molecular weight sulfhydryls: kinetic analysis. (11th Annual Scientific Meeting of the NO-society of Japan, 2011,  Machida, Tokyo, Japan)

5.   Rahaman MM, Sawa T, Okamoto T, Fujii S, Ono K and Akaike T. Structure-activity analysis of nitrated nucleotides as electrophiles for reaction with sulfhydryls. (The 84th Annual Meeting of the Japanese Biochemical Society, September 21-24, 2011, Kyoto, Japan)

6.   Rahaman MM, Sawa T, Okamoto T, Fujii S, Ono K and Akaike T. Proteomic identification for sulfhydryl modifications of mitochondrial proteins by 8-nitroguanosine 3',5'-cyclic monophosphate. (The 6th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric oxide, June14-18, 2010, Kyoto, Japan)

 

  1. Present Research

Currently I am working on different research projects including understanding of different antibiotic resistance mechanisms, novel antibiotics from different natural and innovative sources, Host-pathogen interaction analysis especially activation of inflammasome by bacteria. I am also working on research projects for prevention of coronary artery blockages.

 

  1. Scientific reviewing skills

I have worked as a scientific reviewer for the journal of ‘Nitric Oxide’.

 

  1. Discovery Patents

US Patent of novel small molecule inhibitors of Cytochrome B5 reductase 3 from the Innovation center of University of Pittsburgh.

 

  1. PhD Research

In my graduate study at the Department of Microbiology, Graduate School of Medical Sciences, Kumamoto University, Japan, I had studied the roles of reactive oxygen species (ROS) in the formation of 8-nitroguanosine 3', 5'-cyclic monophosphate (8-nitro-cGMP) and endogenous mitochondrial protein S-guanylation in the cellular milieu under the oxidative stress condition.

 

  1. 8-Nitro-cGMP is a nitrated derivative of cGMP that is formed via cross-talk of reactive oxygen species formed by NADPH oxidase 2 and mitochondria. This nitrated nucleotide can function as a unique electrophilic second messenger in regulation of redox signaling by inducing a post-translational modification of protein thiols via cGMP adduction (protein S-guanylation). With S-guanylation proteomics, I investigated endogenous mitochondrial protein S-guanylation. I had developed a new mass spectrometry (MS)-based proteomic method—S-guanylation proteomics—which comprised two approaches: (i) direct protein digestion followed by immunoaffinity capture of S-guanylated peptides that were subjected to liquid chromatography-tandem MS (LC-MS/MS), and (ii) 2D-gel electrophoretic separation of S-guanylated proteins that were subjected to in-gel digestion, followed by LC-MS/MS. We thereby identified certain mitochondrial proteins that are S-guanylated endogenously during immunological stimulation, including mortalin and 60-kDa heat shock protein (HSP60). Mortalin and HSP60 were recently reported to regulate mitochondrial permeability transition pore (mPTP) opening, at least partly, by interacting with cyclophilin D, an mPTP component. Our data revealed that immunological stimulation and 8-nitro-cGMP treatment induced mPTP opening in a cyclophilin D-dependent manner. Our S-guanylation proteomic method determined that mitochondrial heat shock proteins may be novel targets for redox modification via protein S-guanylation that participates in mPTP regulation and mitochondrial redox signaling.

 

This particular work has been published in Antioxidants and Redox Signaling, 2012 Sep 16 (doi: 10.1089/ars.2012.4606)

 

  1. I have also worked on the electrophilicity of 8-nitro-cGMP and related derivatives with biological low molecular weight sulfhydryls (LMW-SHs) including cysteine, homocysteine and glutathione (GSH) and selenocysteine.  Later I had worked on to determine the effect of these compounds in the inflammation. In brief macrophage derived from php1 monocytes were treated with these compounds followed by stimulated with LPS and IFN gamma to measure the level of different cytokines by real time PCR and also ELISA. This work has been completed and submitted to Journal of Biological Chemistry for publication.

 

  1. One of my major works was to determine enzymatic kinetics of guanylated cyclase.  To determine the kinetic parameters (Km, Vmax and Kcat)  of guanylate cyclase with GTP and modified GTPs for example 8-nitro-GTP, 8-oxo-GTP, 8-Br-GTP I had used 3 different kind of guanylated cyclase including recombinant Chlamydomonas guanylate cyclase, soluble guanylated cyclase from bovine and recombinant particulate guanylated cyclase. I have already finished the study and submitted to Journal of Biological Chemistry for publication.

 

  1. Technical skills in different field

Laser Microscope imaging

  • Confocal laser microscope handling with Nikon EZ-C1

Animal handling

  • Mice handling and small animal surgeries for implantation of osmotic pump, organ isolation, Aorta isolation for myography etc.

Blood Pressure study

  • Pressure myography
  • Animal blood pressure measurement by telemetric assay.

Immunology

-  FACS analysis

-  Western blot analysis

-  ELISA

-  Immunoaffinity purifications of proteins and nanomolecules

-  Immunocytochemistry

-  Immunohistochemistry 

 

Cell biology

- Handling and maintenance of different mammalian cells including, Rat C6 glial cells, Rat smooth muscle cells, Human smooth muscle cells, Endothelial Cells, Mouse Macrophage cells, RAW cells, Hella cells

-  Confocal laser microscope handling (Nikon EZ-C1)

-  Fluorescent microscope handling 


Molecular biology

-           DNA and RNA various preparation methods,

-           PCR, RT-PCR, real time PCR, 


-           Gene silencing using si-RNA, sh-RNA

-           Mammalian cell transfection (lipofectamine based methods)

-           Retroviral gene transfection


-           Apoptosis detecting analyses

Protein-protein interaction

  • Immuno pull down or co-precipitation of proteins.
  • Proximity Ligation Assay (PLA)

Proteomics

-     LC-MS/MS detection of proteins by MASCOT search
- 2D gel electrophoresis
- Silver staining
- Protein isolation from 2D gel and purification for proteomics analysis

Quantitative analysis

-   HPLC- PDA detection and purification of nano molecules

-  HPLC-ECD detection of nanomolecules in subnanomolar level

-  Mass spectrometry for the detection of nanomolecules (Agilent Technologies 6430 Triple Quad)

-  Proteomics analysis by Agilent technology Q-TOF LC/MS

-  Stable isotope quantification of biological materials by LC-MS/MS analysis.

 

Free radical biology and in vitro imaging

-           Reactive oxygen species detection in cells by fluorescent staining, and FACS analysis

-           Mitochondrial superoxide detection by fluorescent imaging

-           Cellular hydrogen peroxide detection by fluorescent method

-           Pegylation of Enzymes, Superoxide dismutase, Catalase

-           Total cell and Mitochondrial SOD activity assay

-           Nitric oxide detection by Griess method 


 

  1. Language Skills

 

          -Bengali: Mother tongue

-English (Reading/Writing/Speaking/Understanding) fluent

          -Japanese (Speaking/Understanding) Fluent

 

  1. Extra curricular activities

 

  • Soccer player of Vascular Medicine Institute, University of Pittsburgh, USA and Department of Microbiology, University of Dhaka, Bangladesh. Also playing cricket in the Department of Microbiology team.
  • Social charity activities during natural disaster situation, i.e., Oral saline project, during flood time in Bangladesh, 1998; During Tsunami in Japan, 2013.
  • Member of nature Study club, Notre-dame College, Dhaka, Bangladesh

 

  1. Hobbies

Photography, Traveling, Playing Cricket, Reading Newspaper, Listening Music, Fishing & Boating.